Coding

Part:BBa_K1321106:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)


Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 206
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 206
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 206
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References